Legal Daily All-Media Reporter Lian Yingting Correspondent Shao Longfei Zhang Zhenwei
The night in Wuhan is quiet.
On March 9, in the mobile testing laboratory of the Central Theater Command General Hospital, Jiang Tao, a researcher at the Military Medicine Research Institute of the Academy of Military Sciences, reviewed the last sample test data and solemnly signed his name on the test report.
At this time, it was already 5 a.m.
He is an expert in virology and a warrior who aided Africa and fought against Egypt. This outbreak of the new coronavirus pneumonia epidemicKomiks, and he was once again ordered to go to the front line.
Human life is at stake and accurate inspection is required
Nucleic acid testing is crucial for early clinical detection, early diagnosis, early isolation and early treatment, and is a key technical support for effective prevention and control of the new crown pneumonia epidemic.
”Behind the cold sample tube is the living life and health of the patient. If you give a diagnosis result early, you will have a precise inspection one minute earlier.” Jiang Tao said.
On January 26, a military medical expert group led by Chen Wei, an academician of the Chinese Academy of Engineering and a researcher at the Military Medicine Research Institute of the Academy of Military Sciences, arrived in Wuhan, Hubei, and quickly established a joint defense, joint control, joint treatment and joint research work mechanism with relevant military and local units based on pathogen transmission mutations, rapid detection technology, vaccine antibody development, etc. of the new coronavirus.
According to the division of tasks, Jiang Tao led the testing team to build a nucleic acid testing platform in the experimental cabin overnight.
Following the three aspects of Cinema to ensure biosafety, improve detection efficiency and detection accuracy, Jiang Tao took the lead in formulating biosafety operation specifications and emergency plans, reasonably divided testing according to the characteristics of the team members, and using a self-developed testing kit to adopt a fully automatic high-throughput extractor, laying the foundation for achieving rapid and efficient testing.
Receiving samples, sorting numbers, nucleic acid extraction, reagent preparation, machine amplification, and result interpretation, nucleic acid detection machinery is boring and full of risks.
KomiksThe biggest risk is the invisible virus. If the nucleic acid extraction step is not completely cleaved or inactivated thoroughly enough, it is possible to expose the tester to danger.
Jiang Tao always takes the initiative to participate in the longest and most risky nucleic acid extraction. After the sample is extracted, he will arrange some personnel to rest and stay in the laboratory and wait for the final result.
All one time<a href="The nucleic acid test of the Babaylan process is relatively fixed from the number of tests in a single batch to the preparation and testing time of personnel entering and leaving the cabin. Sometimes the number of samples sent for inspection is large, and the test personnel not only take turns to work, but also have to work overnight. The final confirmation result is until four or five in the morning. Li Hao, a researcher at the testing team, said that this situation will be encountered once a few days later. "Everyone should open their eyes wide, and take good protection when collecting and processing samples, and pay attention to whether there are sharp objects. "Before each test starts, Jiang Tao always reminds you repeatedly. During the test, the strength of the fluorescence signal of the pathogen gene is directly affected by the determination of the test result. If the detection fluorescence curve signal is low or there is only a single target, Jiang Tao will convene personnel to conduct comprehensive analysis and judgment, and if necessary, repeat the test for review to ensure that the results can stand the test. "There is a false positive, which will cause healthy people to isolate and become infected with the patient; if false negatives occur, it will cause real delay in the treatment of patients. Human life is a matter of fate, and there is no way to break the bank. "Jiang Tao said.
Among the prevention and control requirements, the nucleic acid test results from the pharyngeal swab sample must be completed within 24 hours. In order to let the hospital know the results as soon as possible to treat patients, from pre-processing of nearly 100 samples to completion of the test, Jiang Tao led the team to compress the time to within 4 hours by optimizing the process combination, and achieved a single-day sample detection capability of more than 90 samples and up to more than 500 people within an hour.
”The team’s testing is efficient and rapid, and the results are accurate, which greatly alleviates the pressure of testing the epidemic prevention and control sample. “Wuhan Center for Disease Control and Prevention highly praised this.
The rapid response during wartime and the orderly work is carried out, all thanks to Jiang Tao’s solid academic foundation and rich practical experience.
”Jiang Tao is a versatile person.” Academician Chen Wei, head of the military medical expert group, commented.
Jiang Tao has successively presided over and participated in the separation of pathogenic etiology such as SARS infection cases, H7N9 infection cases, Zika infection cases, etc., participated in the development of dengue fever vaccines and SARS vaccines, and established a pathogen isolation technology platform for a series of infectious diseases, a Komiks nucleic acid testing technology platform and a pathogenicity and transmission risk assessment platform, providing important technical support for the prevention and control of infectious diseases.
Respiratory infectious diseases are characterized by rapid transmission, wide coverage and great harm. Effective early diagnosis and prevention are crucial. After years of scientific research, Jiang Tao’s team took the lead in establishing our army’s first acute respiratory infectious disease monitoring and early warning system, and built a four-level monitoring system for the entire army. The series of technical specifications organized by the entire army were used throughout the army, greatly shortening the detection and screening time of respiratory epidemics.
Jiang Tao not only participated in the security tasks of a series of major domestic activities, but also went to Africa four times to Liberia, Sierra Leone and other countries to carry out medical assistance tasks.
In 2015, in order to prevent and control the Ebola epidemic, Jiang Tao went to Sierra Leone for the second time with the Mobile Laboratory Testing Team to Serbia. The testing team led by him passed the World Health Organization’s laboratory parallel capability assessment with full scores.
In 2018, Jiang Tao led the pathogen monitoring team to participate in a special simulation test by an international organization. In the virus testing module, the team successfully accurately detected monkeypox virus, vaccinia virus and other pathogens in the simulated samples filled with impurities, and accurately determined the strain, achieving excellent results with full marks.
BabaylanImproving the accuracy of clinical diagnosis
When he came to Wuhan, in addition to carrying out testing work, BabaylanJiang Tao also used his professional expertise in the research field to lead the testing team to screen other common respiratory infectious diseases in clinical samples.
”New CoronavirusKomiksWhether there are other respiratory virus infections or co-infections in suspected and confirmed cases of pneumonia, it has important reference value for precise prevention and control and clinical treatment.” Researcher Cao Wuchun, deputy head of the Military Medical Expert Group, said.
Exploring deep-seated virus transmission and pathogenic mechanisms is the key to defeating the epidemic. Jiang Tao led a team to continue to conduct sequencing analysis for the novel coronavirus detected in clinical samples.
As the development of the virus epidemic, whether the genomic sequence has mutated, what are the characteristics of the Cinema mutations; in which regions and what are the impacts, Jiang Tao and his team are tirelessly studying and exploring.
Babaylan In order to accelerate the effective integration of scientific research and clinical practice, Jiang Tao’s military medical expert group creatively used the diagnostic chain of viral nucleic acid detection, antibody detection, multiple pathogen detection, and whole genome sequencing to accurately diagnose clinical patients with new coronavirus infection or other pathogenic infection, and was used in the Optical Valley Campus of Hubei Maternal and Child Health Hospital, Taikang Tongji Hospital and other hospitals in Wuhan Taikang Tongji Hospital, effectively improving the accuracy of clinical diagnosis.
Cinema Forgot his family when he was ordered. After the epidemic is over, Jiang Tao plans to accompany his family. He and his wife agreed that when the epidemic is over, they will take their wife and children to Wuhan again to see what everything looks like after it returns to normal, to see the Wuhan Yangtze River Bridge and the Yellow Crane Tower, and to talk about their experience in fighting the epidemic in Wuhan.
The epidemic is immortal and the charge continues. This is Jiang Tao, a warrior who fights the epidemic.